Filling a gap in the literature of biophysical methodology, this book explains the fundamentals in the theory and practice of AUC. It introduces the basic principles and technical setup of an AUC experiment and describes the optical systems used for detection. It explores the ultracentrifugation experiment from a macromolecular standpoint, offering a detailed physical picture of the sedimentation process and relevant macromolecular parameters. The authors present important practical aspects for conducting an experiment and cover instrument calibration and quality control experiments.
"This state-of-the-art book is very well written and timely. It will be valuable to both experienced and new practitioners in the use of the analytical ultracentrifuge."
-Geoff Howlett, The University of Melbourne
"This eminently readable book tells the outcome - to present date - of a journey of scientific discovery ? Sedimentation analysis of macromolecular systems is the field in which Dr. Peter Schuck and his fellow investigators have been involved throughout the present century and to which they have made contributions not only through their own laboratories' investigations, but through the widespread use of their algorithms and programs by scientists throughout the world. ? this book will be as vital a tool for novices as for senior workers seeking guidance on the more recondite areas of analysis. ? If you already know about sedimentation analysis, you will find interest and information alike in its pages. If you are a newcomer to the area, you should take advantage of the opportunity to read a well-written and authoritative account, and become excited by its possibilities."
-From the Foreword by Arthur J. Rowe, Nottingham
